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analysis lifescience software  (Evident Corporation)

 
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    Evident Corporation analysis lifescience software
    Effect of transplantation of neonatal MPS IIIA mice preconditioned with 4 Gy. (a) The weight of MPS IIIA recipient mice transplanted with normal-GFP donor bone marrow (“MPS IIIA Treated”; n = 7 mice) and transplanted control groups of normal mice receiving normal-GFP cells (“Normal”; n = 9 mice) and MPS IIIA mice receiving MPS IIIA cells (‘MPS IIIA’; n = 5 mice) was recorded at 8 weeks post-treatment. (b) The percentage of donor-derived GFP+CD45+ leukocytes was measured by flow cytometry. (c) The catalytic activity of two proteolytic enzymes that are involved with the mobilization of bone marrow cells into circulation was measured using chromogenic substrates in bone marrow extracellular fluid harvested from untreated normal (open bars) and MPS IIIA (filled bars) mice (n = 9-10 mice per group). (d) Cryo-sections were examined for the presence of donor-derived GFP-expressing cells (green) compared to the total number of DAPI-stained cell nuclei (blue) in the cerebellum. Scale bar is 100 μm. The relative GFP transgene copy number (compare to the HPRT gene as an endogenous control) was determined via quantitative real-time PCR in transplanted mouse (e) brain slice 3 and (f) slice 5. An untreated transgenic normal-GFP mouse brain was used as a calibrating sample (100%). (g) Liver, (h) spleen, and (i) brain SGSH activity was measured in tissue homogenates using a radiolabeled tetrasaccharide substrate and HPLC separation. (j) The relative amount of GlcNS-UA disaccharide was determined by tandem mass spectrometry as a measure of the amount of primary storage material in the transplanted mouse brain. The effect of treatment on GM3 ganglioside storage in the brain was quantitated in the (k) inferior colliculus and (l) caudal cortex using immunohistological methods and AnalySIS <t>Lifescience</t> software. All data are expressed as the mean ± SEM. *P < 0.05, ***P < 0.001 versus the control-transplanted MPS IIIA control group
    Analysis Lifescience Software, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/analysis lifescience software/product/Evident Corporation
    Average 90 stars, based on 1 article reviews
    analysis lifescience software - by Bioz Stars, 2026-04
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    1) Product Images from "Neonatal Bone Marrow Transplantation in MPS IIIA Mice"

    Article Title: Neonatal Bone Marrow Transplantation in MPS IIIA Mice

    Journal: JIMD Reports

    doi: 10.1007/8904_2012_169

    Effect of transplantation of neonatal MPS IIIA mice preconditioned with 4 Gy. (a) The weight of MPS IIIA recipient mice transplanted with normal-GFP donor bone marrow (“MPS IIIA Treated”; n = 7 mice) and transplanted control groups of normal mice receiving normal-GFP cells (“Normal”; n = 9 mice) and MPS IIIA mice receiving MPS IIIA cells (‘MPS IIIA’; n = 5 mice) was recorded at 8 weeks post-treatment. (b) The percentage of donor-derived GFP+CD45+ leukocytes was measured by flow cytometry. (c) The catalytic activity of two proteolytic enzymes that are involved with the mobilization of bone marrow cells into circulation was measured using chromogenic substrates in bone marrow extracellular fluid harvested from untreated normal (open bars) and MPS IIIA (filled bars) mice (n = 9-10 mice per group). (d) Cryo-sections were examined for the presence of donor-derived GFP-expressing cells (green) compared to the total number of DAPI-stained cell nuclei (blue) in the cerebellum. Scale bar is 100 μm. The relative GFP transgene copy number (compare to the HPRT gene as an endogenous control) was determined via quantitative real-time PCR in transplanted mouse (e) brain slice 3 and (f) slice 5. An untreated transgenic normal-GFP mouse brain was used as a calibrating sample (100%). (g) Liver, (h) spleen, and (i) brain SGSH activity was measured in tissue homogenates using a radiolabeled tetrasaccharide substrate and HPLC separation. (j) The relative amount of GlcNS-UA disaccharide was determined by tandem mass spectrometry as a measure of the amount of primary storage material in the transplanted mouse brain. The effect of treatment on GM3 ganglioside storage in the brain was quantitated in the (k) inferior colliculus and (l) caudal cortex using immunohistological methods and AnalySIS Lifescience software. All data are expressed as the mean ± SEM. *P < 0.05, ***P < 0.001 versus the control-transplanted MPS IIIA control group
    Figure Legend Snippet: Effect of transplantation of neonatal MPS IIIA mice preconditioned with 4 Gy. (a) The weight of MPS IIIA recipient mice transplanted with normal-GFP donor bone marrow (“MPS IIIA Treated”; n = 7 mice) and transplanted control groups of normal mice receiving normal-GFP cells (“Normal”; n = 9 mice) and MPS IIIA mice receiving MPS IIIA cells (‘MPS IIIA’; n = 5 mice) was recorded at 8 weeks post-treatment. (b) The percentage of donor-derived GFP+CD45+ leukocytes was measured by flow cytometry. (c) The catalytic activity of two proteolytic enzymes that are involved with the mobilization of bone marrow cells into circulation was measured using chromogenic substrates in bone marrow extracellular fluid harvested from untreated normal (open bars) and MPS IIIA (filled bars) mice (n = 9-10 mice per group). (d) Cryo-sections were examined for the presence of donor-derived GFP-expressing cells (green) compared to the total number of DAPI-stained cell nuclei (blue) in the cerebellum. Scale bar is 100 μm. The relative GFP transgene copy number (compare to the HPRT gene as an endogenous control) was determined via quantitative real-time PCR in transplanted mouse (e) brain slice 3 and (f) slice 5. An untreated transgenic normal-GFP mouse brain was used as a calibrating sample (100%). (g) Liver, (h) spleen, and (i) brain SGSH activity was measured in tissue homogenates using a radiolabeled tetrasaccharide substrate and HPLC separation. (j) The relative amount of GlcNS-UA disaccharide was determined by tandem mass spectrometry as a measure of the amount of primary storage material in the transplanted mouse brain. The effect of treatment on GM3 ganglioside storage in the brain was quantitated in the (k) inferior colliculus and (l) caudal cortex using immunohistological methods and AnalySIS Lifescience software. All data are expressed as the mean ± SEM. *P < 0.05, ***P < 0.001 versus the control-transplanted MPS IIIA control group

    Techniques Used: Transplantation Assay, Derivative Assay, Flow Cytometry, Activity Assay, Expressing, Staining, Real-time Polymerase Chain Reaction, Slice Preparation, Transgenic Assay, Mass Spectrometry, Software



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    Effect of transplantation of neonatal MPS IIIA mice preconditioned with 4 Gy. (a) The weight of MPS IIIA recipient mice transplanted with normal-GFP donor bone marrow (“MPS IIIA Treated”; n = 7 mice) and transplanted control groups of normal mice receiving normal-GFP cells (“Normal”; n = 9 mice) and MPS IIIA mice receiving MPS IIIA cells (‘MPS IIIA’; n = 5 mice) was recorded at 8 weeks post-treatment. (b) The percentage of donor-derived GFP+CD45+ leukocytes was measured by flow cytometry. (c) The catalytic activity of two proteolytic enzymes that are involved with the mobilization of bone marrow cells into circulation was measured using chromogenic substrates in bone marrow extracellular fluid harvested from untreated normal (open bars) and MPS IIIA (filled bars) mice (n = 9-10 mice per group). (d) Cryo-sections were examined for the presence of donor-derived GFP-expressing cells (green) compared to the total number of DAPI-stained cell nuclei (blue) in the cerebellum. Scale bar is 100 μm. The relative GFP transgene copy number (compare to the HPRT gene as an endogenous control) was determined via quantitative real-time PCR in transplanted mouse (e) brain slice 3 and (f) slice 5. An untreated transgenic normal-GFP mouse brain was used as a calibrating sample (100%). (g) Liver, (h) spleen, and (i) brain SGSH activity was measured in tissue homogenates using a radiolabeled tetrasaccharide substrate and HPLC separation. (j) The relative amount of GlcNS-UA disaccharide was determined by tandem mass spectrometry as a measure of the amount of primary storage material in the transplanted mouse brain. The effect of treatment on GM3 ganglioside storage in the brain was quantitated in the (k) inferior colliculus and (l) caudal cortex using immunohistological methods and AnalySIS <t>Lifescience</t> software. All data are expressed as the mean ± SEM. *P < 0.05, ***P < 0.001 versus the control-transplanted MPS IIIA control group
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    Effect of transplantation of neonatal MPS IIIA mice preconditioned with 4 Gy. (a) The weight of MPS IIIA recipient mice transplanted with normal-GFP donor bone marrow (“MPS IIIA Treated”; n = 7 mice) and transplanted control groups of normal mice receiving normal-GFP cells (“Normal”; n = 9 mice) and MPS IIIA mice receiving MPS IIIA cells (‘MPS IIIA’; n = 5 mice) was recorded at 8 weeks post-treatment. (b) The percentage of donor-derived GFP+CD45+ leukocytes was measured by flow cytometry. (c) The catalytic activity of two proteolytic enzymes that are involved with the mobilization of bone marrow cells into circulation was measured using chromogenic substrates in bone marrow extracellular fluid harvested from untreated normal (open bars) and MPS IIIA (filled bars) mice (n = 9-10 mice per group). (d) Cryo-sections were examined for the presence of donor-derived GFP-expressing cells (green) compared to the total number of DAPI-stained cell nuclei (blue) in the cerebellum. Scale bar is 100 μm. The relative GFP transgene copy number (compare to the HPRT gene as an endogenous control) was determined via quantitative real-time PCR in transplanted mouse (e) brain slice 3 and (f) slice 5. An untreated transgenic normal-GFP mouse brain was used as a calibrating sample (100%). (g) Liver, (h) spleen, and (i) brain SGSH activity was measured in tissue homogenates using a radiolabeled tetrasaccharide substrate and HPLC separation. (j) The relative amount of GlcNS-UA disaccharide was determined by tandem mass spectrometry as a measure of the amount of primary storage material in the transplanted mouse brain. The effect of treatment on GM3 ganglioside storage in the brain was quantitated in the (k) inferior colliculus and (l) caudal cortex using immunohistological methods and AnalySIS <t>Lifescience</t> software. All data are expressed as the mean ± SEM. *P < 0.05, ***P < 0.001 versus the control-transplanted MPS IIIA control group
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    Effect of transplantation of neonatal MPS IIIA mice preconditioned with 4 Gy. (a) The weight of MPS IIIA recipient mice transplanted with normal-GFP donor bone marrow (“MPS IIIA Treated”; n = 7 mice) and transplanted control groups of normal mice receiving normal-GFP cells (“Normal”; n = 9 mice) and MPS IIIA mice receiving MPS IIIA cells (‘MPS IIIA’; n = 5 mice) was recorded at 8 weeks post-treatment. (b) The percentage of donor-derived GFP+CD45+ leukocytes was measured by flow cytometry. (c) The catalytic activity of two proteolytic enzymes that are involved with the mobilization of bone marrow cells into circulation was measured using chromogenic substrates in bone marrow extracellular fluid harvested from untreated normal (open bars) and MPS IIIA (filled bars) mice (n = 9-10 mice per group). (d) Cryo-sections were examined for the presence of donor-derived GFP-expressing cells (green) compared to the total number of DAPI-stained cell nuclei (blue) in the cerebellum. Scale bar is 100 μm. The relative GFP transgene copy number (compare to the HPRT gene as an endogenous control) was determined via quantitative real-time PCR in transplanted mouse (e) brain slice 3 and (f) slice 5. An untreated transgenic normal-GFP mouse brain was used as a calibrating sample (100%). (g) Liver, (h) spleen, and (i) brain SGSH activity was measured in tissue homogenates using a radiolabeled tetrasaccharide substrate and HPLC separation. (j) The relative amount of GlcNS-UA disaccharide was determined by tandem mass spectrometry as a measure of the amount of primary storage material in the transplanted mouse brain. The effect of treatment on GM3 ganglioside storage in the brain was quantitated in the (k) inferior colliculus and (l) caudal cortex using immunohistological methods and AnalySIS Lifescience software. All data are expressed as the mean ± SEM. *P < 0.05, ***P < 0.001 versus the control-transplanted MPS IIIA control group

    Journal: JIMD Reports

    Article Title: Neonatal Bone Marrow Transplantation in MPS IIIA Mice

    doi: 10.1007/8904_2012_169

    Figure Lengend Snippet: Effect of transplantation of neonatal MPS IIIA mice preconditioned with 4 Gy. (a) The weight of MPS IIIA recipient mice transplanted with normal-GFP donor bone marrow (“MPS IIIA Treated”; n = 7 mice) and transplanted control groups of normal mice receiving normal-GFP cells (“Normal”; n = 9 mice) and MPS IIIA mice receiving MPS IIIA cells (‘MPS IIIA’; n = 5 mice) was recorded at 8 weeks post-treatment. (b) The percentage of donor-derived GFP+CD45+ leukocytes was measured by flow cytometry. (c) The catalytic activity of two proteolytic enzymes that are involved with the mobilization of bone marrow cells into circulation was measured using chromogenic substrates in bone marrow extracellular fluid harvested from untreated normal (open bars) and MPS IIIA (filled bars) mice (n = 9-10 mice per group). (d) Cryo-sections were examined for the presence of donor-derived GFP-expressing cells (green) compared to the total number of DAPI-stained cell nuclei (blue) in the cerebellum. Scale bar is 100 μm. The relative GFP transgene copy number (compare to the HPRT gene as an endogenous control) was determined via quantitative real-time PCR in transplanted mouse (e) brain slice 3 and (f) slice 5. An untreated transgenic normal-GFP mouse brain was used as a calibrating sample (100%). (g) Liver, (h) spleen, and (i) brain SGSH activity was measured in tissue homogenates using a radiolabeled tetrasaccharide substrate and HPLC separation. (j) The relative amount of GlcNS-UA disaccharide was determined by tandem mass spectrometry as a measure of the amount of primary storage material in the transplanted mouse brain. The effect of treatment on GM3 ganglioside storage in the brain was quantitated in the (k) inferior colliculus and (l) caudal cortex using immunohistological methods and AnalySIS Lifescience software. All data are expressed as the mean ± SEM. *P < 0.05, ***P < 0.001 versus the control-transplanted MPS IIIA control group

    Article Snippet: Images were captured using an Olympus Colorview Soft Imaging System and an Olympus BX41 (GM3 ganglioside) or BX61 (GFP) microscope, and the percentage of immunostained area was determined with AnalySIS Lifescience software (version 2.8, Build1235, Olympus Soft Imaging Solutions).

    Techniques: Transplantation Assay, Derivative Assay, Flow Cytometry, Activity Assay, Expressing, Staining, Real-time Polymerase Chain Reaction, Slice Preparation, Transgenic Assay, Mass Spectrometry, Software